Type

Recombinant

Description

RecombinanthumanFGF2-STABisaproteinFGF2geneticallyengineeredtoenhancethermalstABIlityandprolonghalf-lifewithoutmodificationofaBIOLOGicalfunction.Total175 AA.Mw:19.4 kDa(calculated).N-terminalHis-tagandthrombincleavagerecognitionsite,20 extra AA.

Source

E.coli

Purity

>95%

SDS-PAGEGel

15%SDS-PAGEseparationunderreducingconditions.

1. M.W.Marker –14,18,25,35,4566,116 kDa
2.Recombinantprotein

Endotoxin

<1.0 EU/µg

Formulation

Lyophilizedfromafilteredsolutionin20 mMpotassiumphosphatebufferand750 mMsodiumchloride,pH 7.5.

Reconstitution

Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5–1.0mg/mLandletthelyophilizedpelletdissolvecompletely.

Applications

Cellcultureand/oranimalstudies,Biologicallyactiveprotein

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

Bradfordproteinassaytodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALassaytodeterminequantityofendotoxin.
Proliferationassaywithhumanembryonicstemcells(CCTL14)todeterminebiologicalactivity.

Note

Thisproductisintendedforresearchuseonly.