Type
Recombinant
Description
FGF-basicisoneof23 knownmembersoftheFGFfamily.Proteinsofthisfamilyplayacentralroleduringprenataldevelopment,postnatalgrowthandregenerationofavarietyoftissues,bypromotingcellularproliferationanddifferentiation.FGF-basicisanon-glycosylatedheparinbindinggrowthfactorthatisexpressedinthebrain,pituitary,kidney,retina,bone,testis,adrenalgland,liver,monocytes,epithelialcellsandendothelialcells.FGF-basicsignalsthroughFGFR1b,1c,2c,3cand4. RecombinantMurineFGF-basicisa16.3 kDaproteinconsistingof145 aminoacidresidues.
AminoAcidSequence
PALPEDGGAAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHVKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTEECFFFERLESNNYNTYRSRKYSSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
Source
E.coli
Purity
95%
BIOLOGicalActivity
Assay#1:TheED50asdeterminedbythedose-dependentstimulationofthymidineuptakebyBaF3cellsexpressingFGFreceptorsis≤0.1 ng/mlcorrespondingtoaspecificactivityof≥1×107units/mg.Assay#2:TheED50wasdeterminedbyacellproliferationassayusingbalb/c3T3cellsis≤1.0 ng/ml,correspondingtoaspecificactivityof≥1×106units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
