Type
Recombinant
Description
FGF-basicisoneof23 knownmembersoftheFGFfamily.Proteinsofthisfamilyplayacentralroleduringprenataldevelopment,postnatalgrowthandregenerationofavarietyoftissues,bypromotingcellularproliferationanddifferentiation.FGF-basicisanon-glycosylatedheparinbindinggrowthfactorthatisexpressedinthebrain,pituitary,kidney,retina,bone,testis,adrenalgland,liver,monocytes,epithelialcellsandendothelialcells.FGF-basicsignalsthroughFGFR1b,1c,2c,3cand4. RecombinantHumanFGF-basicisa17.2 kDaproteinconsistingof154 aminoacidresidues.
AminoAcidSequence
AAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
Source
E.coli
Purity
95%
BIOLOGicalActivity
Assay#1:TheED50 asdeterminedbythedose-dependentstimulationofthymidineuptakebyBaF3cellsexpressingFGFreceptorsis≤0.5 ng/ml,correspondingtoaspecificactivityof≥2×106 units/mg.Assay#2:TheED50 wasdeterminedbyacellproliferationassayusingbalb/c3T3cellsis≤0.1 ng/ml,correspondingtoaspecificactivityof≥1×107 units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstitutein5mMTris,pH7.6,toaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
