Type
Recombinant
Description
FGF-acidicisoneof23 knownmembersoftheFGFfamily.Proteinsofthisfamilyplayacentralroleduringprenataldevelopment,postnatalgrowthandregenerationofavarietyoftissues,bypromotingcellularproliferationanddifferentiation.FGF-acidicisanon-glycosylatedheparinbindinggrowthfactorthatisexpressedinthebrain,kidney,retina,smoothmusclecells,bonematrix,osteoblasts,astrocytesandendothelialcells.FGF-acidichastheABIlitytosignalthroughalltheFGFreceptors.RecombinantRatFGF-acidicisa15.9 kDaproteinconsistingof141 aminoacidresidues.
AminoAcidSequence
MFNLPLGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESAGEVYIKGTETGQYLAMDTEGLLYGSQTPNEECLFLERLEENHYNTYTSKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD
Source
E.coli
Purity
95%
BIOLOGicalActivity
Determinedbyacellproliferationassayusingbalb/c3T3cells.TheexpectedED50is≤0.1 ng/ml,inthepresenceof10 µg/mlheparin,correspondingtoaspecificactivityof≥1×107units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstitutein5mMSodiumPhosphate,pH7.2 toaconcentrationof≤0.5 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
