Type

Recombinant

Description

FGF-acidicisoneof23 knownmembersoftheFGFfamily.Proteinsofthisfamilyplayacentralroleduringprenataldevelopment,postnatalgrowthandregenerationofavarietyoftissues,bypromotingcellularproliferationanddifferentiation.FGF-acidicisanon-glycosylatedheparinbindinggrowthfactorthatisexpressedinthebrain,kidney,retina,smoothmusclecells,bonematrix,osteoblasts,astrocytesandendothelialcells.FGF-acidichastheABIlitytosignalthroughalltheFGFreceptors.RecombinantMurineFGF-acidicisa15.9 kDaproteinconsistingof141 aminoacidresidues.

AminoAcidSequence

MFNLPLGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESAGEVYIKGTETGQYLAMDTEGLLYGSQTPNEECLFLERLEENHYNTYTSKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD

Source

E.coli

Purity

95%

BIOLOGicalActivity

Assay#1:TheED₅₀asdeterminedbythedose-dependentstimulationofthymidineuptakeby3T3cellsinthepresenceofheparinis≤0.5 ng/mlcorrespondingtoaspecificactivityof≥2×10⁶units/mg.Assay#2:TheED₅₀wasdeterminedbyacellproliferationassayusingbalb/c3T3cellsis≤0.2 ng/mlinthepresenceof10 μg/mlheparin,correspondingtoaspecificactivityof≥5×10⁶units/mg.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstitutein5mMSodiumPhosphate,pH7.0–7.2 toaconcentrationof0.5–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C