Type
Recombinant
Description
FGF-acidicisoneof23 knownmembersoftheFGFfamily.Proteinsofthisfamilyplayacentralroleduringprenataldevelopment,postnatalgrowthandregenerationofavarietyoftissues,bypromotingcellularproliferationanddifferentiation.FGF-acidicisanon-glycosylatedheparinbindinggrowthfactorthatisexpressedinthebrain,kidney,retina,smoothmusclecells,bonematrix,osteoblasts,astrocytesandendothelialcells.FGF-acidichastheABIlitytosignalthroughalltheFGFreceptors.RecombinantHumanFGF-acidicisa16.8 kDaproteinconsistingof141 aminoacidresidues.
AminoAcidSequence
MFNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD
Source
E.coli
Purity
95%
BIOLOGicalActivity
Assay#1:TheED50 asdeterminedbythedose-dependentstimulationofthymidineuptakebyBaF3cellsexpressingFGFreceptorsis≤10 ng/ml,correspondingtoaspecificactivityof≥1×105 units/mg.Assay#2:TheED50 wasdeterminedbyacellproliferationassayusingbalb/c3T3cellsis≤0.5 ng/mlinthepresenceof10 μg/mlheparin,correspondingtoaspecificactivityof≥2×106 units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstitutein5mMSodiumPhosphate,pH8.0 toaconcentrationof<0.5 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C
Storage/Expiration
–20°C
