Type
Recombinant
Description
FGF-9 isaheparinbindinggrowthfactorthatbelongstotheFGFfamily.Proteinsofthisfamilyplayacentralroleduringprenataldevelopment,postnatalgrowthandregenerationofavarietyoftissues,bypromotingcellularproliferationanddifferentiation.FGF-9 targetsglialcells,astrocytescellsandothercellsthatexpresstheFGFR1c,2c,3b,3c,and4. RecombinantHumanFGF-9 isa23.2 kDaproteinconsistingof206 aminoacidresidues.
AminoAcidSequence
PLGEVGNYFGVQDAVPFGNVPVLPVDSPVLLSDHLGQSEAGGLPRGPAVTDLDHLKGILRRRQLYCRTGFHLEIFPNGTIQGTRKDHSRFGILEFISIAVGLVSIRGVDSGLYLGMNEKGELYGSEKLTQECVFREQFEENWYNTYSSNLYKHVDTGRRYYVALNKDGTPREGTRTKRHQKFTHFLPRPVDPDKVPELYKDILSQS
Source
E.coli
Purity
95%
BIOLOGicalActivity
TheED50 asdeterminedbythedose-dependentstimulationofthymidineuptakebyBaF3cellsexpressingFGFreceptorsis≤0.5 ng/ml,correspondingtoaspecificactivityof≥2 X106 units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
