Type
Recombinant
Description
FGF-8(FGF-8b)isaheparinbindinggrowthfactorbelongingtotheFGFfamily.Proteinsofthisfamilyplayacentralroleduringprenataldevelopment,postnatalgrowthandregenerationofavarietyoftissues,bypromotingcellularproliferationanddifferentiation.Thereare4 knownalternatesplicedformsofFGF8;FGF-8A,FGF-8B,FGF-8EandFGF-8F.ThehumanandmurineFGF-8AandBareidentical,unlikehumanandmouseFGF-8EandF,whichare98%identical.FGF-8 targetsmammarycarcinomacellsandothercellsexpressingtheFGFreceptors.RecombinantHumanFGF-8(FGF-8b)isa22.5 kDaproteinconsistingof194 aminoacidresidues.
AminoAcidSequence
MQVTVQSSPNFTQHVREQSLVTDQLSRRLIRTYQLYSRTSGKHVQVLANKRINAMAEDGDPFAKLIVETDTFGSRVRVRGAETGLYICMNKKGKLIAKSNGKGKDCVFTEIVLENNYTALQNAKYEGWYMAFTRKGRPRKGSKTRQHQREVHFMKRLPRGHHTTEQSLRFEFLNYPPFTRSLRGSQRTWAPEPR
Source
E.coli
Purity
95%
BIOLOGicalActivity
TheED50asdeterminedbythedose-dependentstimulationofthymidineuptakebyBaF3cellsexpressingFGF-receptorsis≤0.5 ng/ml,correspondingtoaspecificactivityof≥2×106units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
