Type

Recombinant

Description

FGF-4 isaheparinbindinggrowthfactorthatisamemberoftheFGFfamily.Proteinsofthisfamilyplayacentralroleduringprenataldevelopment,postnatalgrowthandregenerationofavarietyoftissues,bypromotingcellularproliferationanddifferentiation.FGF-4 signalsthroughtheFGFR1c,2c,3c,and4. RecombinantHumanFGF-4 isa19.7 kDaproteinconsistingof182 aminoacidresidues.

AminoAcidSequence

GRGGAAAPTAPNGTLEAELERRWESLVALSLARLPVAAQPKEAAVQSGAGDYLLGIKRLRRLYCNVGIGFHLQALPDGRIGGAHADTRDSLLELSPVERGVVSIFGVASRFFVAMSSKGKLYGSPFFTDECTFKEILLPNNYNAYESYKYPGMFIALSKNGKTKKGNRVSPTMKVTHFLPRL

Source

E.coli

Purity

95%

BIOLOGicalActivity

TheED₅₀asdeterminedbythedose-dependentstimulationofthymidineuptakebyBaF3cellsexpressingFGF-receptorsis≤0.5 ng/ml,correspondingtoaspecificactivityof≥2×10⁶units/mg.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C