Type

Recombinant

Description

FGF-20 isasecretedheparinbindinggrowthfactorthatisamemberoftheFGFfamily.Proteinsofthisfamilyplayacentralroleduringprenataldevelopment,postnatalgrowthandregenerationofavarietyoftissues,bypromotingcellularproliferationanddifferentiation.FGF-20 signalsthroughtheFGFR2cand3c,andisexpressedduringlimbandbraindevelopment.RecombinantHumanFGF-20 isa23.2 kDaproteincontaining209 aminoacidresidues.

AminoAcidSequence

PLAEVGGFLGGLEGLGQQVGSHFLLPPAGERPPLLGERRSAAERSARGGPGAAQLAHLHGILRRRQLYCRTGFHLQILPDGSVQGTRQDHSLFGILEFISVAVGLVSIRGVDSGLYLGMNDKGELYGSEKLTSECIFREQFEENWYNTYSSNIYKHGDTGRRYFVALNKDGTPRDGARSKRHQKFTHFLPRPVDPERVPELYKDLLMYT

Source

E.coli

Purity

90%

BIOLOGicalActivity

TheED₅₀ asdeterminedbythedose-dependentstimulationofthymidineuptakebyBaF3cellsexpressingFGF-receptorsis10 ng/ml,correspondingtoaspecificactivityof1×10⁵ units/mg.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C