Type
Recombinant
Description
FGF-17 isaheparinbindinggrowthfactorthatisamemberoftheFGFfamily.Proteinsofthisfamilyplayacentralroleduringprenataldevelopment,postnatalgrowthandregenerationofavarietyoftissues,bypromotingcellularproliferationanddifferentiation.FGF-17 signalsthroughtheFGFR1c,2c,3c,and4. FGF-17 signalsinductionandpatterningofembryonicbrain.RecombinantHumanFGF-17 isa22.7 kDaproteinconsistingof195 aminoacidresidues.
AminoAcidSequence
MTQGENHPSPNFNQYVRDQGAMTDQLSRRQIREYQLYSRTSGKHVQVTGRRISATAEDGNKFAKLIVETDTFGSRVRIKGAESEKYICMNKRGKLIGKPSGKSKDCVFTEIVLENNYTAFQNARHEGWFMAFTRQGRPRQASRSRQNQREAHFIKRLYQGQLPFPNHAEKQKQFEFVGSAPTRRTKRTRRPQPLT
Source
E.coli
Purity
95%
BIOLOGicalActivity
Assay#1:TheED50 asdeterminedbythedose-dependentstimulationofthymidineuptakebyBaF3cellsexpressingFGF-receptorsis0.5 ng/ml,correspondingtoaspecificactivityof2×106 units/mg. Assay#2:TheED50 wasdeterminedbyacellproliferationassayusingbalb/c3T3cellsis10.0 ng/ml,correspondingtoaspecificactivityof1×105 units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C
Storage/Expiration
–20°C
