Type
Recombinant
Description
FGF-10 isaheparinbindinggrowthfactorthatbelongstotheFGFfamily.Proteinsofthisfamilyplayacentralroleduringprenataldevelopment,postnatalgrowthandregenerationofavarietyoftissues,bypromotingcellularproliferationanddifferentiation.FGF-10 ismostrelatedtoKGF/FGF-7,andisexpressedduringthedevelopmentand,preferentially,inadultlungs.ItsignalsthroughtheFGFR2b.RecombinantHumanFGF-10 isa19.3 kDaproteinconsistingof170 aminoacidresidues.
AminoAcidSequence
MLGQDMVSPEATNSSSSSFSSPSSAGRHVRSYNHLQGDVRWRKLFSFTKYFLKIEKNGKVSGTKKENCPYSILEITSVEIGVVAVKAINSNYYLAMNKKGKLYGSKEFNNDCKLKERIEENGYNTYASFNWQHNGRQMYVALNGKGAPRRGQKTRRKNTSAHFLPMVVHS
Source
E.coli
Purity
95%
BIOLOGicalActivity
TheED50asdeterminedbythedose-dependentstimulationofthymidineuptakebyBaF3cellsexpressingFGF-receptorsis≤0.5 ng/ml,correspondingtoaspecificactivityof≥2×106units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstitutein5 mMSodiumPhosphate,pH7.4 toaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
