Type
Native
Description
Nativeproteinisolatedfromhumanpooledplasma,349 AA,MW:37.3 kDa(calculatedwithoutglycosylation).ProteinidentityconfirmedbyLC-MS/MS.
Source
Humanplasma
Purity
>95%
SDS-PAGEGel
14%SDS-PAGEseparationofHumanFetuin
1. M.W.Marker –97,66,45,31,21,14 kDa
2.reducedandheatedsample,5μg/lane
3.non-reducedandnon-heatedsample,5μg/lane
Endotoxin
<1.0 EU/ug
Formulation
Filtered(0.4 μm)andlyophilizedfrom0.5 mg/mLsolutionin20 mMTrisbuffer,200 mMNaCl,pH 8.0
Reconstitution
Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Filteryourculturemedia/workingsolutionscontainingthisproductbeforeusingincellculture.
Applications
Westernblotting,ELISA,Cellcultureand/oranimalstudies
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cforthree days.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
Note
AllbloodsamplesusedforproteinpreparationweretestedandfoundnegativeforHBsAg,anti-HCV,anti-HBcandanti-HIV1 and2. Sincenotestcanabsolutelyassuretheabsenceofallinfectiousagents,thisproductshouldbehandledasapotentialbiohazard.Thisproductisintendedforresearchuse only.
