Type

Recombinant

Description

FetuinA/AHSGisahumanplasmaglycoproteinbelongingtotheCystatinfamilyofproteaseinhibitors.Itishighlyexpressedinembryoniccellsandadulthepatocytes,andisexpressedtoalesserextentinmonocytes/macrop­hages.FetuinA/AHSGisamajorserumproteincomponentthatexertsvariouscalcium-dependentphysiologicalactivities,andcanmediategrowthsignalingincertaintumorcells.ItalsocanactasanaturalantagoNISTagainstspecificTGF-βandBMPsignalingproteins,RecombinantHumanFetuinA isaheterodimericproteincontaininga282 aminoacidA chainanda27 aminoacidBchain.ThecalculatedmolecularweightofRecombinantHumanFetuinA is32.9 kDa.Duetoglycosylation,recombinantFetuinA migratesatanapparentmolecularweightof45–55kDabySDS-PAGErunundernon-reducingconditions.

Source

HEK293cells

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytoinhibitCathespinV cleavageofafluorogenicpeptidesubstrateZ-LR-AMC(RLUs). TheexpectedIC₅₀ is 100nM.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C