Type

Recombinant

Description

Recombinantproteincontainsaminoacidsno.2–140oftheproteinacc.no.A6NFH5ofUniProtKB.Total149 AA.MW:16.7 kDa(calculated).N-TerminalHis-tag(10 extra AA)

AminoAcidSequence

MKHHHHHHASIDQLQGTWKSISCENSEDYMKELGIGRASRKLGRLAKPTVTISTDGDVITIKTKSIFKNNEISFKLGEEFEEITPGGHKTKSKVTLDKESLIQVQDWDGKETTITRKLVDGKMVVESTVNSVICTRTYEKVSSNSVSNS

Source

E.coli

Purity

Purityasdeterminedbydensitometricimageanalysis: >95%

SDS-PAGEGel

14%SDS-PAGEseparationofHumanFABP12
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,2.5μg/lane
3.non-reducedandnon-heatedsample,2.5μg/lane

Endotoxin

<1.0 EU/ug

Formulation

Filtered(0.4 μm)andlyophilizedfrom0.5 mg/mLin20mMTrisbuffer,50 mMNaCl,pH 7.5

Reconstitution

Add200µlofdeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mlandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA,Immunoassays

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.