Type

Recombinant

Description

Total207 AA.MW:23.1 kDa(calculated).N-TerminalHis-tag(10 extraAA).UniprotKBacc.no.P98173.ProteinidentityconfirmedbyLC-MS/MS.IdentificationofrecombinantFAM3AbyLC-MS/MS.

AminoAcidSequence

MKHHHHHHASFPRIQQLFTSPESSVTAAPRARKYKCGLPQPCPEEHLAFRVVSGAANVIGPKICLEDKMLMSSVKDNVGRGLNIALVNGVSGELIEARAFDMWAGDVNDLLKFIRPLHEGTLVFVASYDDPATKMNEETRKLFSELGSRNAKELAFRDSWVFVGAKGVQNKSPFEQHVKNSKHSNKYEGWPEALEMEGCIPRRSTAS

Source

E.coli

Purity

Purityasdeterminedbydensitometricimageanalysis:> 95%

SDS-PAGEGel

12%SDS-PAGEseparationofHumanFAM3A:
1. M.W.Marker –97,66,45,31,21,14 kDa
2.reducedandboiledsample,2.5μg/lane
3.non-reducedandnon-boiledsample,2.5μg/ lane

Endotoxin

<1.0 EU/µg

Formulation

Filtered(0.4 μm)andlyophilizedin0.5 mg/mLin100mMTRISpH7.5 containing0.1Marginine.

Reconstitution

Add200µlofdeionizedwatertoprepareaworkingstocksolutionof0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.

Applications

Westernblotting,ELISA

Shipping

Attemperature2 –8°C.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cforthree days.

QualityControlTest

Bradfordproteinassaytodeterminequantityoftheprotein.
SDS-PAGEtodeterminepurityoftheprotein.
LALTESTtodetermineendotoxin level.

Note

Thisproductisintendedforresearchuseonly.