Type

Recombinant

Description

Selectinsareafamilyofcalcium-dependenttype1 transmembraneproteins.Endothelial(E)-selectinisaheavilyglycosylatedtransmembraneproteinexpressedbyactivatedendothelialcellsinmicrovascularlinings.E-selectin,alongwithP-selectinandL-selectin,initiaterecruitmentofcirculatingleukocytesfrombloodtositesofinflammationinthevascularliningthroughinteractionwithspecificcellsurface-associatedcarbohydratedeterminants.E-selectinconsistsofanN-terminaltype1 lectindomain,anEGF-likedomain,6 sushi(CCP/SCR)domains,atransmembranesequence,andashortcytoplasmicdomain.RecombinantHumanE-selectinisa58.6 kDaproteincontaining535 aminoacidresidues,correspondingtotheextracellularportionofthefulllengthprotein.Duetoglycosylation,E-selectinmigratesatanapparentmolecularweightofapproximately65–85kDabySDS-PAGEanalysisunderreducingconditions.

AminoAcidSequence

WSYNTSTEAMTYDEASAYCQQRYTHLVAIQNKEEIEYLNSILSYSPSYYWIGIRKVNNVWVWVGTQKPLTEEAKNWAPGEPNNRQKDEDCVEIYIKREKDVGMWNDERCSKKKLALCYTAACTNTSCSGHGECVETINNYTCKCDPGFSGLKCEQIVNCTALESPEHGSLVCSHPLGNFSYNSSCSISCDRGYLPSSMETMQCMSSGEWSAPIPACNVVECDAVTNPANGFVECFQNPGSFPWNTTCTFDCEEGFELMGAQSLQCTSSGNWDNEKPTCKAVTCRAVRQPQNGSVRCSHSPAGEFTFKSSCNFTCEEGFMLQGPAQVECTTQGQWTQQIPVCEAFQCTALSNPERGYMNCLPSASGSFRYGSSCEFSCEQGFVLKGSKRLQCGPTGEWDNEKPTCEAVRCDAVHQPPKGLVRCAHSPIGEFTYKSSCAFSCEEGFELHGSTQLECTSQGQWTEEVPSCQVVKCSSLAVPGKINMSCSGEPVFGTVCKFACPEGWTLNGSAARTCGATGHWSGLLPTCEAPTESNIP

Source

ChineseHamsterOvaryCells(CHO)

Purity

95%

BIOLOGicalActivity

MeasuredbyitsABIlitytosupportadhesionofU937cells,ahumanhystiocyticlymphomacell line.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C