Type

Recombinant

Description

GlialderivedNeurotrophicFactorHumanRecombinantproducedinE.coliisahomodimer,non-glycosylated,polypeptidechaincontaining2×135aminoacidsandhavingatotalmolecularmassof30,360 Dalton.GDNFispurifiedbyproprietarychromatographictechniques.

AminoAcidSequence

ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeMet-Ser-Pro-Asp-Lys.

Source

E.coli

Purity

Greaterthan98.0%asdeterminedby:(a)AnalysisbyRP-HPLC.©AnalysisbySDS-PAGE.

BIOLOGicalActivity

TheED50,calculatedbythedose-dependantdopaminuptakeinratmesencephaliccultureswasfoundtobe5–10 ng/ml.

Formulation

GDNFwaslyophilizedafterdialysisagainst10 mMsodiumcitrateand150 mM NaCl.

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

LyophilizedGlial-derivedNeurotrophicFactoralthoughstableatroomtemperaturefor3 weeks,shouldbestoreddesiccatedbelow –18°C.UponreconstitutionGDNFshouldbestoredat4°Cbetween2–7daysandforfutureusebelow –18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.

PhysicalAppearance

Sterilefilteredwhitelyophilized(freeze-dried)powder.