Description

GHHumanRecombinantproducedinE.coliisasingle,non-glycosylated,polypeptidechaincontaining192 aminoacidsandhavingamolecularmassof22260.01 Dalton.GrowthHormoneispurifiedbyproprietarychromatographictechniques.

AminoAcidSequence

ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeMet-Phe-Pro-Thr-Ile.

Source

E.coli

Purity

Greaterthan98.0%asdeterminedby(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.

BIOLOGicalActivity

Biovendor’s GHiscontains3 units/mg.

Formulation

TheGH(1 mg/ml)waslyophilizedafterextensivedialysesagainst8 mgmannitoland0.34 mgsodiumphosphatebuffer(0.02 mgsodiumphosphatemonobasic&0.32 mgsodiumphosphatedibasic).

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

LyophilizedGHalthoughstableatroomtemperaturefor3 weeks,shouldbestoreddesiccatedbelow –18°C.UponreconstitutionHGHshouldbestoredat4°Cbetween2–7daysandforfutureusebelow –18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.

PhysicalAppearance

Sterilefilteredwhitelyophilized(freeze-dried)powder.