Type
Recombinant
Description
EpigenisanEGF-relatedpolypeptidegrowthfactorthatsignalsthroughtheErbBreceptor-1. Itisproducedinseveraltissues,includingthetestis,liver,andheart,aswellasincertaintumorcells.Epigenismitogenicforfibroblastsandepithelialcells.HumanEpigenisinitiallysynthesizedasaglycosylated14.7 kDatransmembraneprecursorprotein,whichisprocessedbyproteolyticcleavagetoproduceamaturesolublesequence.RecombinantHumanEpigenisa7.9 kDamonomericprotein,containing72 aminoacidresidues,whichcomprisestheEGF-homologousportionoftheEpigenprecursor.
AminoAcidSequence
AVTVTPPITAQQADNIEGPIALKFSHLCLEDHNSYCINGACAFHHELEKAICRCFTGYTGERCEHLTLTSYA
Source
E.coli
Purity
98%
BIOLOGicalActivity
Determinedbythedose-dependentstimulationoftheproliferationofmurineBalb/3T3cells. TheexpectedED50forthiseffectis150–300ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C
Storage/Expiration
–20°C
