Description

RecombinantHumanEGFproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining53 aminoacidsandhavingamolecularmassof6222 Dalton.TherHuEGFispurifiedbyproprietarychromatographictechniques.

AminoAcidSequence

ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeAsn-Ser-Asp-Ser-Glu,whichagreeswiththesequenceofnativehumanEGF.N-terminalmethioninehasbeencompletelyremovedenzymatically.

Source

E.coli

Purity

Greaterthan98.0%asdeterminedby:-(a)AnalysisbyRP-HPLC.-(b)Anion-exchangeFPLC.-©Analysisbyreducingandnon-reducingSDS-PAGESilverStainedgel.Dimersandaggregates:Lessthan1%asdeterminedbysilver-stainedSDS-PAGEgelanalysis.

BIOLOGicalActivity

Biovendor´s re­combinanthumanEGFisfullybiologicallyactivewhencomparedtostandards.TheED50,calculatedbythedose-dependantproliferationofmurineBALB/c3T3cells(measuredby³H-thymidineuptake)is<0.1 ng/mlcorrespondingtoaspecificactivityof1×10⁷Units/mg.

Endotoxin

Lessthan0.1 ng/µg(IEU/µg)ofrHuEGF.

Formulation

Theproteinwaslyophilizedfromaconcentrated(1mg/mL)solutionwithnoadditives.

Reconstitution

ItisrecommendedtoreconstitutethelyophilizedrHuEGFinsterile18MΩ-cmH2O notlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

LyophilizedrHuEGFalthoughstableatroomtemperaturefor3 weeks,shouldbestoreddesiccatedbelow –18°C.UponreconstitutionrHuEGFshouldbestoredat4°Cbetween2–7daysandforfutureusebelow –18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.

PhysicalAppearance

Sterilefilteredwhitelyophilized(freeze-dried)powder.