Type

Recombinant

Description

Total170 AA.MW:20.2 kDa(calculated).UniProtKBacc.No.P20827(Asp19–Ser182).C-terminalHis-tag(6 extraAA).ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

DRHTVFWNSSNPKFRNEDYTIHVQLNDYVDIICPHYEDHSVADAAMEQYILYLVEHEEYQLCQPQSKDQVRWQCNRPSAKHGPEKLSEKFQRFTPFTLGKEFKEGHSYYYISKPIHQHEDRCLRLKVTVSGKITHSPQAHDNPQEKRLAADDPEVRVLHSIGHSHHHHHH

Source

HEK293

Purity

Purityasdeterminedbydensitometricimageanalysis:>95 %

SDS-PAGEGel

14 %SDS-PAGEseparationofHumanEFNA1(HEK):
1. M.W.Marker –14,21,31,45,66,97 kDa
2.Reducedandboiledsample,2.5 μg/lane
3.Non-reducedandnon-boiledsample,2.5 μg/lane

Endotoxin

<1.0 EU/μg

Formulation

Filtered(0.4 μm)andlyophilizedfrom0.5 mg/mlsolutioninphosphatebufferedsalinepH7.5 containing5 %(w/v)trehalose.

Reconstitution

Add200 µlofdeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mlandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

Note

Thisproductisintendedforresearchuseonly.