Type

Recombinant

Description

Eotaxin-3 isaCCchemokinethatsignalsthroughtheCCR3receptor.ItisproducedbyendothelialcellsstimulatedwithIL-4 orIL-13. Eotaxin-3 selectivelytargetscellsexpressingCCR3,includingeosinophils,basophils,Tcellsandmonocytes.Eotaxin-3 hassimilaractivitytoEotaxinandEotaxin-2,butthethreeEotaxinsshareonlyalowdegreeofsequencehomology.RecombinantHumanEotaxin-3 isan8.4 kDaproteincontaining71 aminoacidresidues,includingthefourhighlyconservedcysteineresiduespresentinCCchemokines.

AminoAcidSequence

TRGSDISKTCCFQYSHKPLPWTWVRSYEFTSNSCSQRAVIFTTKRGKKVCTHPRKKWVQKYISLLKTPKQL

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattracthumanCCR3/HEK293 cells. Themaximumactivitywasachievedat2 ug/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C