Type

Recombinant

Description

Eotaxin-2 isaCCchemokinethatsignalsthroughtheCCR3receptor.ItisproducedbyactivatedmonocytesandTlymphocytes.Eotaxin-2 selectivelychemoattractscellsexpressingCCR3,includingeosinophils,basophils,Th4Tcells,mastcells,andcertainsubsetsofdendriticcells.Additionally,Eotaxin-2 inhibitstheproliferationofmultipotentialhematopoieticProgenitorcells.Thematureprotein,whichalsoincludesaC-terminaltruncation,contains78 aminoacidresidues(92 a.a.residuesforthemurinehomolog,withoutC-terminaltruncation).Eotaxin-2 containsthefourconservedcysteineresiduespresentinCCchemokines.RecombinantMurineEotaxin-2 isa10.3 kDaproteincontaining93 aminoacidresidues.

AminoAcidSequence

VTIPSSCCTSFISKKIPENRVVSYQLANGSICPKAGVIFITKKGHKICTDPKLLWVQRHIQKLDAKKNQPSKGAKAVRTKFAVQRRRGNSTEV

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattractmurinelymphocytesusingaconcentrationrangeof10–100 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C