Type

Recombinant

Description

EotaxinisaCCchemokinethatsignalsthroughtheCCR3receptor.ItisproducedbyIFN-γ-stimulatedendothelialcellsandTNF-activatedmonocytes.Eotaxinselectivelychemoattractseosinophilsand,alongwithEotaxin-2 andEotaxin-3,playsakeyroleintheregulationofeosinophilrecruitmentintheasthmaticlungandinallergicreactions.RecombinantMurineEotaxinisan8.4 kDaproteincontaining74 aminoacidresidues,includingthefourhighlyconservedcysteineresiduespresentinCCchemokines.

AminoAcidSequence

HPGSIPTSCCFIMTSKKIPNTLLKSYKRITNNRCTLKAIVFKTRLGKEICADPKKKWVQDATKHLDQKLQTPKP

Source

E.coli

Purity

98%

BIOLOGicalActivity

MurineEotaxinwasfoundtoinducechemotaxisofpurifiedeosinophilsatconcentrationsrangingbetween100–1000 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C