Type

Recombinant

Description

Proteases(alsocalledProteolyticEnzymes,Peptidases,orProteinases)areenzymesthathydrolyzetheamidebondswithinproteinsorpeptides.Mostproteasesactinaspecificmanner,hydrolyzingbondsat,oradjacentto,specificresidues,oraspecificsequenceofresiduescontainedwithinthesubstrateproteinorpeptide.ProteasesplayanimportantroleinmostdiseasesandBIOLOGicalprocesses,includingprenatalandpostnataldevelopment,reproduction,signaltransduction,theimmuneresponse,variousautoimmuneanddegenerativediseases,andcancer.Theyarealsoanimportantresearchtool,frequentlyusedintheanalysisandproductionofproteins.EnterokinasesequentiallycleavescarboxylsideofD-D-D-D-K.HumanEnterokinaseisexpressedasalinear1019 aminoacidpolypeptideprecursorglycoprotein.ProteolyticprocessingofthisprecursorgeneratesthebiologicallyactiveformofEnterokinase,whichconsistsoftwopolypeptidechains(heavychainandlightchain)heldtogetherbyasingledisulfidebond,resultinginformationofabiologicallyactiveheterodimer.Theheavychainconsistsof784 aminoacidresidues,andthelightchainconsistsof235 aminoacidresidues.ThecalculatedmolecularweightofRecombinantHumanEnterokinaseis108.7 kDa.

AminoAcidSequence

Heavychain:LTIKESQRGAALGQSHEARATFKITSGVTYNPNLQDKLSVDFKVLAFDLQQMIDEIFLSSNLKNEYKNSRVLQFENGSIIVVFDLFFAQWVSDQNVKEELIQGLEANKSSQLVTFHIDLNSVDILDKLTTTSHLATPGNVSIECLPGSSPCTDALTCIKADLFCDGEVNCPDGSDEDNKMCATVCDGRFLLTGSSGSFQATHYPKPSETSVVCQWIIRVNQGLSIKLSFDDFNTYYTDILDIYEGVGSSKILRASIWETNPGTIRIFSNQVTATFLIESDESDYVGFNATYTAFNSSELNNYEKINCNFEDGFCFWVQDLNDDNEWERIQGSTFSPFTGPNFDHTFGNASGFYISTPTGPGGRQERVGLLSLPLDPTLEPACLSFWYHMYGENVHKLSINISNDQNMEKTVFQKEGNYGDNWNYGQVTLNETVKFKVAFNAFKNKILSDIALDDISLTYGICNGSLYPEPTLVPTPPPELPTDCGGPFELWEPNTTFSSTNFPNSYPNLAFCVWILNAQKGKNIQLHFQEFDLENINDVVEIRDGEEADSLLLAVYTGPGPVKDVFSTTNRMTVLLITNDVLARGGFKANFTTGYHLGIPEPCKADHFQCKNGECVPLVNLCDGHLHCEDGSDEADCVRFFNGTTNNNGLVRFRIQSIWHTACAENWTTQISNDVCQLLGLGSGNSSKPIFSTDGGPFVKLNTAPDGHLILTPSQQCLQDSLIRLQCNHKSCGKKLAAQDITPKLightChain:IVGGSNAKEGAWPWVVGLYYGGRLLCGASLVSSDWLVSAAHCVYGRNLEPSKWTAILGLHMKSNLTSPQTVPRLIDEIVINPHYNRRRKDNDIAMMHLEFKVNYTDYIQPICLPEENQVFPPGRNCSIAGWGTVVYQGTTANILQEADVPLLSNERCQQQMPEYNITENMICAGYEEGGIDSCQGDSGGPLMCQENNRWFLAGVTSFGYKCALPNRPGVYARVSRFTEWIQSFLH

Source

ChineseHamsterOvaryCells(CHO)

Purity

90%

BiologicalActivity

SequentiallycleavescarboxylsideofD-D-D-D-K.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C