Type

Recombinant

Description

ENA-78 isaCXCchemokinethatsignalsthroughtheCXCR2receptor.Itisexpressedinmonocytes,platelets,endothelialcells,andmastcells.ENA-78 isachemoattractantforneutrophils.ThethreenaturallyoccurringvariantsofhumanENA-78;ENA5–78,ENA9–78andENA10–78,contain74,70,and69 aminoacidresidues,respectively,andpossessthesameBIOLOGicalactivity.ENA-78 containsthefourconservedcysteineresiduespresentinCXCchemokines,andalsocontainsthe‘ELR’motifcommontoCXCchemokinethatbindtotheCXCR1andCXCR2receptors.RecombinantHumanENA-78 isan8.0 kDaproteinconsistingof74 aminoacidresidues.

AminoAcidSequence

AAVLRELRCVCLQTTQGVHPKMISNLQVFAIGPQCSKVEVVASLKNGKEICLDPEAPFLKKVIQKILDGGNKEN

Source

E.coli

Purity

98%

BiologicalActivity

DeterminedbyitsABIlitytochemoattracthumanperipheralbloodneutrophilsusingaconcentrationof5.0–10.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C