Type

Recombinant

Description

EGFisapotentgrowthfactorthatstimulatestheproliferationofvariousepidermalandepithelialcells.Additionally,EGFhasbeenshowntoinhibitgastricsecretion,andtobeinvolvedinwoundhealing.EGFsignalsthroughareceptorknownasc-erbB,whichisaclassI tyrosinekinasereceptor.ThisreceptoralsobindswithTGF-αandVGF(vacciniavirusgrowthfactor).RecombinantMurineEGFisa6.0 kDaglobularproteincontaining53 aminoacidresidues,including3 intramoleculardisulfide bonds.

AminoAcidSequence

NSYPGCPSSYDGYCLNGGVCMHIESLDSYTCNCVIGYSGDRCQTRDLRWWELR

Source

E.coli

Purity

98%

BIOLOGicalActivity

TheED₅₀wasdeterminedbyacellproliferationassayusingBALB/c3T3cellsis≤0.1 ng/ml,correspondingtoaspecificactivityof≥1×10⁷units/mg.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.5–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C