Type
Recombinant,Animal-Freemanufactured
AminoAcidSequence
NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR
Source
E.coli
Purity
>98%bySDS-PAGEgellandHPLCanalysis
BIOLOGicalActivity
DeterminedbyitsABIlitytostimulatetheproliferationofmousebalb/c3T3cells.TheED50is≤0.1 ng/ml,correspondingtoaspecificactivityof≥1×107units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example5%Trehalose)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
