Type

Recombinant, Animal-Free manufactured

Amino Acid Sequence

NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR

Source

E. coli

Purity

>98% by SDS-PAGE gell and HPLC analysis

Biological Activity

Determined by its ability to stimulate the proliferation of mouse balb/c 3T3 cells. The ED₅₀ is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1×10⁷ units/mg.

Endotoxin

Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1–1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 5% Trehalose) and store in working aliquots at –20°C to –80°C.

Storage/Expiration

 –20°C