Biovendor/Cysteine-Rich Secretory Protein 3 Human E. coli/RD172262100/0.1 mg-免疫在线蚂蚁淘旗下平台

商品详细Biovendor/Cysteine-Rich Secretory Protein 3 Human E. coli/RD172262100/0.1 mg

Biovendor/Cysteine-Rich Secretory Protein 3 Human E. coli/RD172262100/0.1 mg

商品编号： RD172262100

品牌： Biovendor-Laboratorni

市场价： ¥6700.00

美元价： 4020.00

产地：美国(厂家直采)

公司：

产品分类：细胞因子

公司分类： cytokine

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Regulatorystatus:RUO
Type:Recombinant
Source:E.coli
Othernames:CRISP-3,SpecificGranuleProtein(28KDa),CRS3,SGP28,Aeg2
Species:Human

Type

Recombinant

Description

Recombinantproteincontainsaminoacidsno.21–245oftheproteinacc.no.P54108inUniProtKB.Total235 AA.MW:26.74 kDa(calculated).N-TerminalHis-tag(10 extra AA)

AminoAcidSequence

MKHHHHHHASNEDKDPAFTALLTTQTQVQREIVNKHNELRRAVSPPARNMLKMEWNKEAAANAQKWANQCNYRHSNPKDRMTSLKCGENLYMSSASSSWSQAIQSWFDEYNDFDFGVGPKTPNAVVGHYTQVVWYSSYLVGCGNAYCPNQKVLKYYYVCQYCPAGNWANRLYVPYEQGAPCASCPDNCDDGLCTNGCKYEDLYSNCKSLKLTLTCKHQLVRDSCKASCNCSNSIY

Source

E.coli

Purity

Purityasdeterminedbydensitometricimageanalysis:> 85%

SDS-PAGEGel

14 %SDS-PAGEseparationofHumanCysteine-richsecretoryprotein3:
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandboiledsample,2.5 μg/lane
3.non-reducedandnon-boiledsample,2.5 μg/lane

Endotoxin

<1.0 EU/ug

Formulation

0.3 –0.5 mg/mlin50 mMacetatebuffer,pH4,5mMDTT20%glycerol. –filtered(0.4 μm), frozen

Reconstitution

Defrostatambienttemperature.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA

Shipping

Onice.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storeproteinat –80°C.Proteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Avoidrepeatedfreezing/thawingcycles.

QualityControlTest

Bradfordproteinassaytodeterminequantityoftheprotein.
SDS-PAGEtodeterminepurityoftheprotein.
LALTESTtodetermineendotoxin level.

Note

Thisproductisintendedforresearchuseonly.

品牌介绍

miREIA是一种基于免疫测定的新型miRNA定量方法，涉及从患者样品中分离出的miRNA与互补的生物素化DNA寡核苷酸探针杂交。然后通过固定有完美匹配的DNA / miRNA杂交体的微量滴定板固定的单克隆抗体捕获DNA / RNA杂交体。下一步是复制标准ELISA方案。该测定可在常规免疫测定设备上运行，与标准临床工作流程兼容，不需要扩增步骤，并且包括miRNA分析在内的结果可在不到三个小时的时间内获得。