Type

Recombinant

Description

Total134 AA.Mw:15 kDa(calculated).UniProtKBacc.no.P21460.N-terminalHis-tag,14 extraAA(highlighted).

AminoAcidSequence

MRGSHHHHHHGMASATPKQGPRMLGAPEEADANEEGVRRALDFAVSEYNKGSNDAYHSRAIQVVRARKQLVAGVNYFLDVEMGRTTCTKSQTNLTDCPFHDQPHLMRKALCSFQIYSVPWKGTHSLTKFSCKNA

Source

E.coli

Purity

>95%

SDS-PAGEGel

12%SDS-PAGEseparationofMouseCystatinC
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,5μg/lane
3.non-reducedandnon-heatedsample,5μg/lane

Endotoxin

<1.0 EU/μg

Formulation

Filtered(0.4 μm)andlyophilizedin0.5 mg/mLin20mMTrisbuffer,50 mMNaCl,pH 7.5

Reconstitution

Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.