Type
Native
Description
Nativeproteinisolatedfromhumanurine.
Source
Humanurine
Purity
Purityasdeterminedbydensitometricimageanalysis: >96%
SDS-PAGEGel
12%SDS-PAGEseparationofHumanCystatinC
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,5μg/lane
3.non-reducedandnon-heatedsample,5μg/lane
Endotoxin
<1.0 EU/ug
Formulation
Filtered(0,4 μm))andlyophilisedfrom0.6 mg/mLin20mMNH4HCO3withtracesofbuffer salts.
Reconstitution
Add20mMNH4HCO3toprepareaworkingstocksolutionofapproximately0.5 mg/mL.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
Applications
Westernblotting,ELISA
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
Note
Thisproductisintendedforresearchuseonly.AllstartingmaterialusedforpurificationwastestedandfoundnegativeforHIV1 and2 antibodies,HepatitisBsurfaceantigenandHCVantibodies.