Type

Recombinant

Description

Total129 AA.Mw:14.5 kDa(calculated).UniProtKBacc.no.P01034.N-terminalHis-tag,9 extraAA(highlighted).

AminoAcidSequence

MKHHHHHHASSPGKPPRLVGGPMDASVEEEGVRRALDFAVGEYNKASNDMYHSRALQVVRARKQIVAGVNYFLDVELGRTTCTKTQPNLDNCPFHDQPHLKRKAFCSFQIYAVPWQGTMTLSKSTCQDA

Source

E.coli

Purity

Purityasdeterminedbydensitometricimageanalysis: >95%

SDS-PAGEGel

12%SDS-PAGEseparationofHumanCystatinC
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,2.5μg/lane
3.non-reducedandnon-heatedsample,2.5μg/lane

Endotoxin

<1.0 EU/ug

Formulation

Filtered(0,4 μm)andlyophilizedfrom0.5 mg/mLin20mMTrisbuffer,50 mMNaCl,pH 7.5

Reconstitution

Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4°Cforalimitedperiodoftime;howeveritdoesshowtraceofdegradation(below5%oftotalproduct)afteroneweekat 4°C.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.