Type
Recombinant
Description
CYR61isamemberoftheCCNfamilyofsecretedcysteine-richregulatoryproteins.CYR61inducesangiogenesisbystimulatingtheproliferation,migration,andadhesionofendothelialcells.Cellmigrationandadhesionaremediatedthroughbindingtospecificcellsurfaceintegrinsandtoheparinsulfateproteoglycans.IncreasedexpressionofCYR61isassociatedwithseveraltypesofcancer,andcorrelateswiththeprogressionandestrogenindependenceofhumanbreastcancers.RecombinantHumanCYR61isa39.5 kDaproteincontaining357 aminoacidresidues.Itiscomposedoffourdistinctstructuraldomains(modules);theIGFbindingprotein(IGFBP)domain,thevonWillebrandFactorC(VWFC)domain,theThrombospondintype-I(TSPtype-1)domain,andaC-terminalcysteineknot-likedomain(CTCK).
AminoAcidSequence
MTCPAACHCPLEAPKCAPGVGLVRDGCGCCKVCAKQLNEDCSKTQPCDHTKGLECNFGASSTALKGICRAQSEGRPCEYNSRIYQNGESFQPNCKHQCTCIDGAVGCIPLCPQELSLPNLGCPNPRLVKVTGQCCEEWVCDEDSIKDPMEDQDGLLGKELGFDASEVELTRNNELIAVGKGSSLKRLPVFGMEPRILYNPLQGQKCIVQTTSWSQCSKTCGTGISTRVTNDNPECRLVKETRICEVRPCGQPVYSSLKKGKKCSKTKKSPEPVRFTYAGCLSVKKYRPKYCGSCVDGRCCTPQLTRTVKMRFRCEDGETFSKNVMMIQSCKCNYNCPHANEAAFPFYRLFNDIHKFRD
Source
E.coli
Purity
95%
BIOLOGicalActivity
Determinedbythedose-dependentstimulationoftheproliferationofmurine3T3cells.TheexpectedED50forthiseffectis2.0–3.0 µg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
