Type
Recombinant
Description
CXCL16isamemberoftheCXCchemokinefamilyandsignalsthroughtheCXCR6receptor.CXCL16mayplayaroleinattractinglymphocytesubsetsduringinflammationandmayfacilitatecertainimmuneresponses.ThechemokinedomainofCXCL16containssixcysteineresidues,includingthefourhighlyconservedcysteineresiduescharacteristicofCXCchemokines.TheCXCL16genecodesfora273 aminoacidpolypeptide,whichincludesa29 aminoacidcytoplasmicdomainandtransmembranesequencecontainingapproximately20 aminoacids.TheextracellularportionofCXCL16containsachemokinesdomainandanextendedC-terminalmucin-likestalksequence.Theextracellulardomaincontains89 aminoacidresidues(86 a.a.residuesforthemurinehomolog).RecombinantMurineCXCL16isa9.9 kDaproteincontaining88 aminoacidresidues.
AminoAcidSequence
NQGSVAGSCSCDRTISSGTQIPQGTLDHIRKYLKAFHRCPFFIRFQLQSKSVCGGSQDQWVRELVDCFERKECGTGHGKSFHHQKHLP
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytochemoattractmurinelymphocytesusingaconcentrationof20–1000 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
