Type

Recombinant

Description

Total68 AA.MW:8,0 kDa(calculated).UniProtKBacc.no#P480612(24–89)ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

KPVSLSYRCPCRFFESHVARANVKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNK

Source

E.coli

Purity

PurityasdeterminedbySDSPAGE:>97%

BIOLOGicalActivity

EC50=0.31nMdeterminedbyCalciumFluxwithhumanCXCR4inU937cellsEC50=0.17nMdeterminedbyMigrationwithU937cellsSPABindingassayofCXCL12/SDF-1αtoCXCR7(IC50=14pM)andCXCR4(IC50= 2nM)

Endotoxin

<0.01 EU/1µg

Reconstitution

Addsterilewatertoprepareaworkingstocksolutionofapproximately0.1 mg/mlandletthelyophilizedpelletdissolvecompletely.

Applications

Westernblotting,ELISA,Cellcultureand/oranimalstudies,Biologicallyactiveprotein

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –20to –70°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat-20 to –70°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat -20 to –70°C,theproteinisstablefor3 monthsat –20to –70°Cundersterileconditionsafterreconstitution.Reconstitutedproteincanbestoredat4 °Cfor1 monthundersterileconditionsafterreconstitution

QualityControlTest

QuantificationisdonebyA280,usingthecalculatedextinctioncoefficient(8730 M-1 cm-1).
SDS-PAGEtodeterminepurityoftheprotein.
LALTESTtodetermineendotoxin level.

Note

Thisproductisintendedforresearchuseonly.