Type
Recombinant
Description
Total87 AA.MW:10,4 kDa(calculated).BiotinylatedatthelastLysineinsequence.UniProtKBacc.no#P480612(24–89)ProteinidentityconfirmedbyLC-MS/MS.
AminoAcidSequence
KPVSLSYRCPCRFFESHVARANVKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNKKLGSGLNDIFEAQKIEWHE
Source
E.coli
Purity
PurityasdeterminedbySDSPAGE:>97%
BIOLOGicalActivity
EC50=2.5nMdeterminedbyCalciumFluxwithhumanCXCR4inU937cells.
MigrationconfirmedwithU937cellsexpressing CXCR4
Endotoxin
<0.01 EU/1µg
Reconstitution
Addsterilewatertoprepareaworkingstocksolutionofapproximately0.1 mg/mlandletthelyophilizedpelletdissolvecompletely.
Applications
Westernblotting,Immunocytochemistry,ELISA,Cellcultureand/oranimalstudies,Biologicallyactiveprotein,Flowcytometry
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –20to –70°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat-20 to –70°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat -20 to –70°C,theproteinisstablefor3 monthsat –20to –70°Cundersterileconditionsafterreconstitution.Reconstitutedproteincanbestoredat4 °Cfor1 monthundersterileconditionsafterreconstitution
QualityControlTest
QuantificationisdonebyA280,usingthecalculatedextinctioncoefficient(14,420 M-1 cm-1).
SDS-PAGEtodeterminepurityoftheprotein.
LALTESTtodetermineendotoxin level.
Note
Thisproductisintendedforresearchuseonly.
