Type
Recombinant
Description
CTGFL/WISP-2 isa28.6 kDaproteinthatbelongstotheCCNfamilyofcysteine-richregulatoryproteins.Membersofthisfamilystimulatemitosis,adhesion,apoptosis,extracellularmatrixproduction,growtharrest,andmigrationofmultiplecelltypes.Theproteinisexpressedinprimaryosteoblasts,fibroblasts,theovaries,testes,andheart.Inadditiontopromotingadhesionofosteoblasts,CTGFL/WISP-2 inhibitsosteocalcinproduction,aswellasbindingoffibrinogentointegrinreceptors.RecombinantHumanCTGFL/WISP-2 isa24.3 kDaproteinconsistingof228 aminoacidresidues.MaturehumanCTGFL/WISP-2 isa24.8 kDapolypeptideproteincontaining227 aminoacids.Itiscomposedof3 distinctdomains;theIGF-BindingProteindomain(IGF-BP),theThrombospondintypeI repeat(TSPtypeI),andvonWillebrandFactorCmotif(VWFC).
AminoAcidSequence
MQLCPTPCTCPWPPPRCPLGVPLVLDGCGCCRVCARRLGEPCDQLHVCDASQGLVCQPGAGPGGRGALCLLAEDDSSCEVNGRLYREGETFQPHCSIRCRCEDGGFTCVPLCSEDVRLPSWDCPHPRRVEVLGKCCPEWVCGQGGGLGTQPLPAQGPQFSGLVSSLPPGVPCPEWSTAWGPCSTTCGLGMATRVSNQNRFCRLETQRRLCLSRPCPPSRGRSPQNSAF
Source
E.coli
Purity
95%
BIOLOGicalActivity
TheED50wasdeterminedbyitsABIlitytoinhibitIGF-IIinducedproliferationofMCF-7 isbetween10–20ng/mlinthepresenceof15 ng/mlofhumanIGF-II.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Note:DonotraisetoneutralpH.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C
Storage/Expiration
–20°C
