Type
Recombinant
Description
CTGFisamemberoftheCCNfamilyofsecretedcysteine-richregulatoryproteins,andisthemajormitogenicandchemoattractantproteinproducedbyumbilicalveinandvascularendothelialcells.CTGFstimulatestheproliferationanddifferentiationofchondrocytes,inducesangiogenesis,promotescelladhesionoffibroblasts,endothelialandepithelialcells,andbindstoIGF,TGFβ1andBMP-4. CellmigrationandadhesionaresignaledthroughbindingtospecificcellsurfaceintegrinsandtoheparinsulfateproteoglycansCTGF(98 a.a.),alowermolecularweightisoformcontainingtheC-terminalportionofthefulllengthCTGFprotein,exertsfullheparinbinding,celladhesion,andmitogenicCTGFactivity.MatureHumanCTGFisa38.0 kDasecretedproteincontaining323 aminoacidresidues.CTGFiscomprisedoffourdistinctstructuraldomains(modules),whichareidentifiedasIGFbindingprotein(IGFBP),vonWillebrandFactorC(VWFC),thrombospondintype-I(TSPtype-I),andC-terminalcysteineknot-like(CTCK)domains.FulllengthCTGFcanbeproteolyticallycleavedincertaintissuestoyieldN-terminaltruncatedisoforms,which,dependingontheisoform,containonlytheTSPtype-I andCTCKdomainsorcontainonlytheCTCKdomain.RecombinantHumanCTGFisan11.0 kDaproteinconsistingof97 aminoacidresidues.
AminoAcidSequence
GKKCIRTPKISKPIKFELSGCTSMKTYRAKFCGVCTDGRCCTPHRTTTLPVEFKCPDGEVMKKNMMFIKTCACHYNCPGDNDIFESLYYRKMYGDMA
Source
E.coli
Purity
Greaterthan95%bySDS-PAGEgelandHPLCanalyses.
BIOLOGicalActivity
Determinedbythedose-dependentstimulationoftheproliferationofHUVECcells.TheexpectedED50forthiseffectis1.0–2.0 µg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/µgofprotein(<1EU/µg ).
Formulation
Sterilefilteredthrougha0.2 micronfilter.Lyophilizedfrom10mMSodiumAcetate,pH 6.0
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(eg,0.1%BSA)andstoreat –20to –80 °C.
Storage/Expiration
–20°C
QualityControlTest
VerifiedbyUVSpectroscopyand/orSDS-PAGEgel.
Note
Thisproductisintendedforresearchuseonly.
