Type

Recombinant

Description

CTACKisakeratinocyte-derivedCCchemokinewhichsignalsthroughtheCCR10receptor.BothCTACKandCCR10areexpressedinnormalandirritatedepithelialcells.CTACKselectivelyattractsCLA+T-cellsanddirectsthemintotheskin.CTACKcontainsthefourhighlyconservedcysteineresiduespresentinmostCCchemokines.Thematureproteincontains88 aminoacidresidues.RecombinantMurineCTACKisa10.9 kDaproteincontaining95 aminoacidresidues.

AminoAcidSequence

LPLPSSTSCCTQLYRQPLPSRLLRRIVHMELQEADGDCHLQAVVLHLARRSVCVHPQNRSLARWLERQGKRLQGTVPSLNLVLQKKMYSNPQQQN

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattracthumanperipheralbloodlymphocytesusingaconcentrationrangeof10.0–100.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C