Type

Recombinant

Description

CTACKisakeratinocyte-derivedCCchemokinewhichsignalsthroughtheCCR10receptor.BothCTACKandCCR10areexpressedinnormalandirritatedepithelialcells.CTACKselectivelyattractsCLA+T-cellsanddirectsthemintotheskin.CTACKcontainsthefourhighlyconservedcysteineresiduespresentinmostCCchemokines.Thematureproteincontains88 aminoacidresidues.RecombinantHumanCTACKisa10.2 kDaproteincontaining88 aminoacidresidues.

AminoAcidSequence

FLLPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQRSICIHPQNPSLSQWFEHQERKLHGTLPKLNFGMLRKMG

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattractCXCR3transfectedcellsusingaconcentrationof10.0–100.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C