Type

Recombinant

Description

Total308 AA.MW:34.58 kDa(calculated).UniProtKBacc.no.P24387.N-TerminalHis-tag,10 extraAA(highlighted).

AminoAcidSequence

MKHHHHHHASYLELREAADYDPFLLFSANLKRELAGEQPYRRALRCLDMLSLQGQFTFTADRPQLHCAAFFISEPEEFITIHYDQVSIDCQGGDFLKVFDGWILKGEKFPSSQDHPLPSAERYIDFCESGLSRRSIRSSQNVAMIFFRVHEPGNGFTLTIKTDPNLFPCNVISQTPNGKFTLVVPHQHRNCSFSIIYPVVIKISDLTLGHVNGLQLKKSSAGCEGIGDFVELLGGTGLDPSKMTPLADLCYPFHGPAQMKVGCDNTVVRMVSSGKHVNRVTFEYRQLEPYELENPNGNSIGEFCLSGL

Source

E.coli

Purity

Purityasdeterminedbydensitometricimageanalysis:>90 %

SDS-PAGEGel

14%SDS-PAGEseparationofHumanCRFBP
1. M.W.Marker –97,66,45,31,21,14 kDa
2.reducedandboiledsample,2μg/lane
3.non-reducedandnon-boiledsample,2μg/lane

Endotoxin

<1.0 EU/μg

Formulation

Filtered(0.4 μm)andlyophilizedin0.5 mg/mLin20mMTRIS,50mMNaCl, pH 7.5

Reconstitution

Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletelyatleastfor15 minutesat37°C.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.