Type

Recombinant

Description

Total329 AA,UniProtP29279(Gln27-Ala349).MW:36 kDa(calculated).C-terminal6×Histag.ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

QNCSGPCRCPDEPAPRCPAGVSLVLDGCGCCRVCAKQLGELCTERDPCDPHKGLFCHFGSPANRKIGVCTAKDGAPCIFGGTVYRSGESFQSSCKYQCTCLDGAVGCMPLCSMDVRLPSPDCPFPRRVKLPGKCCEEWVCDEPKDQTVVGPALAAYRLEDTFGPDPTMIRANCLVQTTEWSACSKTCGMGISTRVTNDNASCRLEKQSRLCMVRPCEADLEENIKKGKKCIRTPKISKPIKFELSGCTSMKTYRAKFCGVCTDGRCCTPHRTTTLPVEFKCPDGEVMKKNMMFIKTCACHYNCPGDNDIFESLYYRKMYGDMAHHHHHH

Source

HEK293

Purity

Purityasdeterminedbydensitometricimageanalysis: >90%

SDS-PAGEGel

12%SDS-PAGEseparationofHumanCTGFHEK293:
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,2.5μg/lane
3.non-reducedandnon-heatedsample,2.5μg/ lane

Endotoxin

<1.0 EU/µg

Formulation

Sterilefiltered(0.2 μm)andfrozenfrom0.10 –0.30mg/mLsolutionincitratebufferpH4,7 containing20%(w/v)glycerol.

Reconstitution

Defrostatambienttemperature

Applications

Westernblotting,ELISA,Cellcultureand/oranimalstudies

Shipping

Onice.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storeproteinat –80°C.Proteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Avoidrepeatedfreezing/thawingcycles.Thethawedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.