Type

Recombinant

Description

Total255 AA.MW:28,4 kDa(calculated).UniProtKBacc.no.P60827.N-TerminalHis-tag,10 extraAA(highlighted).

AminoAcidSequence

MKHHHHHHASWPGLPRRPCVHCCRPAWPPGPYARVSDRDLWRGDLWRGLPRVRPTIDIEILKGEKGEAGVRGRAGRSGKEGPPGARGLQGRRGQKGQVGPPGAACRRAYAAFSVGRRAYAAFSVGRREGLHSSDHFQAVPFDTELVNLDGAFDLAAGRFLCTVPGVYFLSLNVHTWNYKETYLHIMLNRRPAAVLYAQPSERSVMQAQSLMLLLAAGDAVWVRMFQRDRDNAIYGEHGDLYITFSGHLVKPAAEL

Source

E.coli

SDS-PAGEGel

14%SDS-PAGEseparationofHumanCTRP8
1. reducedandheatedsample,5μg/lane
2.M.W.Marker –14,21,31,45,66,97 kDa
3.non-reducedandnon-heatedsample,5μg/ lane

Endotoxin

<1.0 EU/μg

Formulation

0.2–0.6 mg/mlin0.03Macetatebuffer,pH=4.0 –filtered(0.4 μm), frozen

Reconstitution

Defrostatambienttemperature.ForconversionintohigherpHvalue,werecommendintensivedilutionbyrelevantbuffertoaconcentrationof10μg/mL.Inhigherconcentrationsthesolubilityofthisantigenislimited.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting

Shipping

Onice.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storeproteinat –80°C.Proteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Avoidrepeatedfreezing/thawingcycles.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.