Type

Recombinant

Description

Total242 AA.MW:27.02 kDa(calculated).UniProtKBacc.no.Q9BXI9.N-terminalHis-tag,10extraAA(highlighted).

AminoAcidSequence

MKHHHHHHASTFDRAVASGCQRCCDSEDPLDPAHVSSASSSGRPHALPEIRPYINITILKGDKGDPGPMGLPGYMGREGPQGEPGPQGSKGDKGEMGSPGAPCQKRFFAFSVGRKTALHSGEDFQTLLFERVFVNLDGCFDMATGQFAAPLRGIYFFSLNVHSWNYKETYVHIMHNQKEAVILYAQPSERSIMQSQSVMLDLAYGDRVWVRLFKRQRENAIYSNDFDTYITFSGHLIKAEDD

Source

E.coli

Purity

Purityasdeterminedbydensitometricimageanalysis:>95 %

SDS-PAGEGel

12%SDS-PAGEseparationofHumanCTRP6:
1. M.W.Marker –14,21,31,45,66,97 kDa
2.non-reducedandnon-heatedsample,2.5μg/lane
3.reducedandheatedsample,2.5μg/ lane

Endotoxin

<1.0 EU/ug

Formulation

0.2 –0.6 mg/mlin0.03Macetatebuffer,5mMDTTpH=4.0 –filtered(0.4 μm), frozen

Reconstitution

Defrostatambienttemperature.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA

Shipping

Onice.Uponreceipt,storetheproductatthetemperaturerecommendedbelow.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storeproteinat –80°C.Proteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Avoidrepeatedfreezing/thawingcycles.

QualityControlTest

Bradfordproteinassaytodeterminequantityoftheprotein.
SDS-PAGEtodeterminepurityoftheprotein.
LALTESTtodetermineendotoxin level.

Note

Thisproductisintendedforresearchuseonly.