Type

Recombinant

Description

CNTFisapotentneuralfactorthatwasoriginallycharacterizedasavitalfactorforthesurvivalofchickciliaryneuronsinvitro.CNTFisalsoimportantforthesurvivalofotherneuralcelltypes,includingprimarysensoryneurons,motorneurons,basalforebrainneuronsandtype2 astrocytes.CNTFishighlyconservedacrossspeciesandexhibitscross-speciesbioactivity.RecombinantRatCNTFissynthesizedasa199 aminoacidpolypeptide(22.7 kDa)lackingahydrophobicN-terminalsignalforsecretion.

AminoAcidSequence

AFAEQTPLTLHRRDLCSRSIWLARKIRSDLTALMESYVKHQGLNKNINLDSVDGVPVASTDRWSEMTEAERLQENLQAYRTFQGMLTKLLEDQRVHFTPTEGDFHQAIHTLMLQVSAFAYQLEELMVLLEQKIPENEADGMPATVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRVISSHQMGISALESHYGAKDKQM

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytostimulateproliferationofhumanTF-1 cellsusingaconcentrationrangeof25.0–35.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C