Type
Recombinant
Description
CNTFisapotentneuralfactorthatwasoriginallycharacterizedasavitalfactorforthesurvivalofchickciliaryneuronsinvitro.CNTFisalsoimportantforthesurvivalofotherneuralcelltypes,includingprimarysensoryneurons,motorneurons,basalforebrainneuronsandtype2 astrocytes.CNTFishighlyconservedacrossspeciesandexhibitscross-speciesbioactivity.RecombinantHumanCNTFissynthesizedasa199 aminoacidpolypeptide(22.8 kDa)lackingahydrophobicN-terminalsignalforsecretion.
AminoAcidSequence
AFTEHSPLTPHRRDLCSRSIWLARKIRSDLTALTQSYVKHQGLNKNINLDSADGMPVASTDQWSQLTQAQRLQQNLQAYRTFHVLLARLLQDQQVHFTPTQGDFHQAIHTLLLQVAAFAYQIQQLMILLQYKIPRNQADGMPINVGDGGLFQKKLWGLKVLQQLSQWTVRSIHDLRFISSHQTGIPARGSHYIANNKKM
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytostimulateproliferationofhumanTF-1 cellsusinganED50concentrationrangeof50–150 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstitutein5–10mMSodiumPhosphate,pH7.5 toaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C
Storage/Expiration
–20°C
