Type
Native
Description
Nativeproteinpurifiedfromhumanplasma
Source
Plasma
Purity
Purityasdeterminedbydensitometricimageanalysis: >95%
SDS-PAGEGel
12%SDS-PAGEseparationofHumanClusterin
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,5μg/lane
3.non-reducedandnon-heatedsample,5μg/lane
Endotoxin
<1.0 EU/ug
Formulation
Filtered(0.4 μm)andlyophilizedin0.5 mg/mLinPBSbuffer,pH 7.5
Reconstitution
Adddeionizedwatertoprepareaworkingstocksolutionof0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
Applications
Westernblotting,ELISA,Cellcultureand/oranimalstudies
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
Note
Materialusedforproteinpreparationwastestedandfoundnegativeforanti-HCVandHIVAg/Ab.Sincenotestcanabsolutelyassuretheabsenceofallinfectiousagents,thisproductshouldbehandledasapotentialbiohazard.Thisproductisintendedforresearchuseonly.
