Type

Recombinant

Description

Total80 AA.MW:9.2 kDa(calculated).N-TerminalHis-tag(10 extraAA).UniprotKBacc.no. P11684

AminoAcidSequence

MKHHHHHHASEICPSFQRVIETLLMDTPSSYEAAMELFSPDQDMREAGAQLKKLVDTLPQKPRESIIKLMEKIAQSSLCN

Source

E.coli

Purity

Purityasdeterminedbydensitometricimageanalysis: >90%

SDS-PAGEGel

14%SDS-PAGEseparationofClubCellProtein
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandboiledsample,2.5μg/lane
3.non-reducedandnon-boiledsample,2.5μg/lane

Endotoxin

<1.0 EU/ug

Formulation

Filtered(0.4 μm)andlyophilizedin0.5 mg/mLin0.05 Mphosphatebuffer,0.075 MNaCl,pH 7.4

Reconstitution

Add200μlofdeionizedwatertoprepareaworkingstocksolutionof0.5 mg/mlandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.

Applications

Westernblotting,ELISA

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.